MUC1 Antibody (MUC1/845) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18143389	Novus Biologicals NBP2-47881-0.1MG	0.1 mg

Description

MUC1 Monoclonal specifically detects MUC1 in Human samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: MUC-1
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cellular Markers, Extracellular Matrix
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 4582
• Immunogen: Human milk-fat globule membranes (HMFGM)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: MUC1/845
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.1 - 0.2 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, CD227, CD227 antigen, DF3 antigen, EMA, episialin, H23 antigen, H23AG, KL-6, MAM6, MUC-1, MUC1/ZD, mucin 1, cell surface associated, mucin 1, transmembrane, mucin-1, Peanut-reactive urinary mucin, PEMMUC-1/SEC, PEMT, Polymorphic epithelial mucin, PUMMUC-1/X, tumor associated epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
• Gene Symbols: MUC1
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.