mTOR Monoclonal Antibody (F11), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 14-2190-80

Product Code. 15247317

Description

Description: This F11 monoclonal antibody reacts with human, mouse, and rat mammalian target of rapamycin (mTOR). This 289-kDa serine/threonine kinase is activated via phosphorylation at Ser2448 by the PI3K/Akt signaling pathway in response to growth factors, energy, nutrients, and stress. mTOR is highly conserved amongst eukaryotes and constitutively expressed in immune (e.g. T, B, NK, dendritic cells, mast cells, and neutrophils) and non-immune tissues (e.g. heart). mTOR exists as a member of two distinct complexes, mTORC1 and mTORC2, which have differing sensitivities to rapamycin and phosphorylation targets. Rapamycin inhibits mTOR activity by binding FKBP12, which causes dissociation of mTORC1 and mTORC2. This molecule plays a critical role in cell metabolism, survival, dendritic cell maturation and activation, and protein synthesis. Deregulation of mTOR has been implicated in a variety of cancers. Studies have also suggested a role for mTOR in Foxp3 expression and regulatory T cell differentiation. Applications Reported: This F11 antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of paraffin embedded tissue sections. Applications Tested: This F11 antibody has been tested by western blot analysis of reduced cell lysates prepared from Jurkat cells. This can be used at 1-5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation of 4EBP1, and initiation of mRNA translation. A second pathway involves regulation of ribosomal S6 kinase, which affects ribosome biogenesis and translation elongation. Mutations affecting the gene results in Smith-Kingsmore syndrome.

Specifications

• Antigen: mTOR
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P42345, Q9JLN9
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: F11
• Conjugate: Unconjugated
• Gene: MTOR
• Gene Symbols: MTOR
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2475, 56717
• Content And Storage: 4° C
• Form: Liquid

For Research Use Only.