MP Biomedicals™ Proteinase K from Tritirachium album

Product Code. 11466391

Brand: MP Biomedicals™ 0219398125

Description

Highly active stable endopeptidase with a broad spectrum of action

Proteinase K is used in digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA, as well as in removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A and in determination of enzyme localization on membranes.

• Grade: Molecular biology reagent
• Specific Activity: >30 mAnson u/mg
• Presentation: White powder
• Isoelectric Point: 8.9 (Lit.)
• Extinction Coefficient: E1%= 14.2 (280 nm,10 mM NaCl and 5 mM CaCl2, pH 8.0) (Lit.)
• pH: 7.5 -12.00 (denatured hemoglobin as substrate) (Lit.)
• Soluble in water

One Anson unit is the amount of enzyme which will liberate 1 °mole of folin-positive amino acids per minnute at pH 7.5 and 35°C using hemoglobin as substrat

Isolation of DNA and RNA

Specifications

• Color: White
• Grade: Molecular Biology
• pH: 7.5 to 12
• For Use With (Application): Useful for the proteolytic inactivation of Nucleases during the isolation of DNA and RNA
• Content And Storage: 4°C
• Source: Tritirachium album
• Quantity: 25 mg
• Product Type: Proteinase K
• Molecular Weight (g/mol): 30000
• Inhibitors: Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
• Form: Lyophilized Powder

Safety and Handling

Product Identifier

• Proteinase K, Molecular Biology Reagent

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P321-Specific treatment (see on this label).
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Proteinase K