Invitrogen™ Mouse VISTA/B7-H5/PD-1H ELISA Kit

Product Code. 17125679

Brand: Invitrogen™ EM74RBX5

Description

ELISA

Mouse VISTA/B7-H5/PD-1H quantitates mouse VISTA/B7-H5/PD-1H in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse VISTA/B7-H5/PD-1H.

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

Specifications

• Accession Number: 4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; DD1alpha; Death Domain1alpha; Dies1; differentiation of ESCs 1; GI24; PD-1 H; PDCD1 homolog; PD-H1; platelet receptor Gi24; PP2135; RIKEN cDNA 4632428N05 gene; SISP1; sisp-1; Stress-induced secreted protein-1; UNQ730/PRO1412; V-domain Ig suppressor of T cell activation; VISTA; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; Vsir; V-type immunoglobulin domain-containing suppressor of T-cell activation
• Assay Range: 3 to 600 ng/mL
• Assay Sensitivity: 3 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 74048
• Gene Symbol: VSIR
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: VISTA
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins