Invitrogen™ VCAM-1 Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMVCAM1

Product Code. 15445353

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Mouse VCAM-1 ELISA quantitates Ms VCAM-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms VCAM-1. Principle of the method The Mouse VCAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

VCAM-1 (vascular cell adhesion protein 1, CD106, INCAM-110) is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases. VCAM-1 is distributed across non-leukocyte and leukocyte cells. VCAM-1 expression is reportedly correlated with adhesion of melanoma, Burkitt lymphoma, osteosarcoma, and kidney carcinoma but not colon carcinoma cells to endothelial cells which may determine the site of tumor metastasis. VCAM-1 is a member of the Ig superfamily of adhesion molecules, is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on unstimulated endothelial cells. VCAM-1 is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. VCAM-1 serves as a ligand for leukocyte integrin alpha-beta (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. VCAM-1 plays a role in tumor metastasis, and may also be involved in the development of atherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms of VCAM-1 have been described.

Specifications

• Accession Number: P29533
• Assay Sensitivity: 1.5 pg/mL
• Description: VCAM-1 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Streptavidin-HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of VCAM-1 in Samples Using a Microplate Reader
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Protein Family: Other Proteins
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 bet
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD106,Vcam-1
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-mouse VCAM-1 precoated 96-well strip plate, lyophilized recombinant mouse VCAM-1 standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X concentr
• Quantity: 96 Tests
• Research Discipline: Development
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 2.05-500 pg/mL
• Calibration Range: 1.5pg/mL to 500pg/mL
• Detectable Analytes: VCAM-1
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 Assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 22329
• Host Species: Mouse
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
• Synonym: CD106, Vcam-1
• Test Time: 1 hr. 20 min.