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Invitrogen™ TNFSF4 (OX40L) Mouse ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EMTNFSF4
Code : NEW
Additional Details : Weight : 0.00400kg
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
The Mouse TNFSF4 (OX40L) ELISA quantitates Ms TNFSF4 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TNFSF4. Principle of the method The Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.Specifications
P43488 | |
30 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
22164 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Inflammation | |
2°C to 8°C | |
Mouse | |
4 hr. 45 min. |
32.77-8000 pg/mL | |
30pg/mL to 8000pg/mL | |
Biotin | |
Cytokines & Receptors | |
This ELISA pair antibody detects mouse TNFSF4/OX40 Ligand and on cross reactivity with rmBAFF, rmEDA, rmGITR Ligand, rmFas Ligand, rmLIGHT, rmTWEAK, rhAPRIL, or rhTNFSF4. | |
Mouse | |
Colorimetric Microplate Reader | |
Ath-1,Ath1,CD134L,OX-40L,Ox40l,TXGP1,Tnlg2b,Txgp1l,gp34 | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
Ath-1, Ath1, CD134L, OX-40L, Ox40l, TXGP1, Txgp1l, gp34 | |
1 hr. 20 min. |