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Thermo Scientific™ Pierce™ Mouse TNF alpha ELISA Kits

Measure levels of mouse tumor necrosis factor alpha (TNFa) in mouse serum and cell culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ Pierce™ EMTNFA2

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10067093

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Description

Description

These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse TNFα (mouse tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 50 to 2450pg/mL

  • Sensitivity: < 9pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNα or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Specifications

Specifications

50 to 2450pg/mL
Mouse TNF alpha ELISA Kit
Complete ELISA kit with coated 96-well plates
Absorbance at 450nm minus absorbance at 550nm
<9pg/mL
50┬ÁL
2 x 96 microplate wells
Natural and recombinant mouse TNFa (mouse tumor necrosis factor alpha)
30X Wash Buffer, 2 x 50mL
Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Intra-assay CV <10%; inter-assay CV <10%
2 plate kit
Cell culture supernatant, serum
No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNa or human TNFa. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFa in culture supernatants
3 hr.
Documentation

Technical Documents