Invitrogen™ Th1/Th2 Mouse Uncoated ELISA Kit

Uncoated ELISA Kit

Brand:  Invitrogen™ 88-7711-44

Product Code. 15521177

Includes:

• Capture Antibody: Pre-titrated, purified antibody
• Detection Antibody. Pre-titrated, biotin-conjugated antibody
• Standard. Recombinant cytokine for generating standard curve and calibrating samples
• ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
• Assay Diluent. 5X concentrated
• Detection enzyme. Pre-titrated Avidin-HRP
• Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

The Mouse Th1/Th2 Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse Th1/Th2 proteins (Interferon gamma, IL-2, IL-4 and IL-10). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

T helper type 1 (Th1) cells and T helper type 2 (Th2) cells are both a lineage of CD4 effector T cells. Th1 and Th2 are involved in promoting cell-mediated immunity. Th1 cells secrete IFN-gamma, IL-2, IL-10, and TNF-alpha/beta, which are required for host defense against intracellular viral and bacterial pathogens. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL-17E/IL-15, which are required for humoral immunity and play an important role in coordinating the immune response to larger extracellular pathogens.

Specifications

• Accession Number: P01580,P04351,P07750,P18893
• Assay Sensitivity: IFN-gamma: 15 pg/mL; IL-2: 2 pg/mL; IL-4: 4 pg/mL; IL-10: 30 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IFG,IFI
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 24 hr. 30 min.

• Assay Range: IFN-gamma: 15-2,000 pg/mL; IL-2: 2-200 pg/mL; IL-4: 4-500 pg/mL; IL-10: 30-4,000 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 15978, 16153, 16183, 16189
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
• Quantity: 96 Tests
• Sample Volume: Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
• Synonym: IFNg, IL-10, IL-2, IL-4
• Test Time: 1 hr. 45 min.

For Research Use Only