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Invitrogen™ pro-MMP-9 Mouse ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EMMMP9
Code : NEW
Additional Details : Weight : 0.00400kg
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
The Mouse pro-MMP-9 ELISA quantitates Ms proMMP-9 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms proMMP-9. Principle of the method The Mouse proMMP-9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.Specifications
P41245 | |
50 pg/mL | |
Pro-MMP-9 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Streptavidin-HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of pro-MMP-9 in Samples Using a Microplate Reader | |
Solid-phase sandwich ELISA | |
Colorimetric | |
Proteases, Peptidases & Inhibitors | |
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, | |
Mouse | |
Colorimetric Microplate Reader | |
AW743869,B/MMP9,Clg4b,Gel B,MMP-9,pro-MMP-9 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-mouse pro-MMP-9 precoated 96-well strip plate, lyophilized recombinant mouse pro-MMP-9 standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X co | |
96 Tests | |
Bone Research | |
2°C to 8°C | |
Mouse | |
4 hr. 45 min. |
74.07-18,000 pg/mL | |
50pg/mL to 18000pg/mL | |
Pro-MMP-9 | |
Biotin | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
96 Assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
17395 | |
Mouse | |
<10% | |
HRP | |
RUO | |
Plasma, 2 μL; Serum, 2 μL; Supernatant, 100 μL | |
RP23-61O3.10-001, AW743869, B/MMP9, Clg4b, MMP-9, pro-MMP-9 | |
1 hr. 20 min. |