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Invitrogen™ pro-MMP-9 Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMMMP9

Code : NEW

Additional Details : Weight : 0.33000kg

Product Code. 15475313

  • £398.00 / 1 set


EU Stock 1
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Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.
Specifications

Specifications

P41245
Colorimetric Microplate Reader
74.07-18,000 pg/mL
Biotin
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-mouse pro-MMP-9 precoated 96-well strip plate, lyophilized recombinant mouse pro-MMP-9 standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X co
1 Kit
50 pg/mL
Plasma, 2 μL|Serum, 2 μL|Supernatant, 100 μL
2°C to 8°C
1 hr. 20 min.
Mouse
Pro-MMP-9
Proteases, Peptidases & Inhibitors
AW743869,B/MMP9,Clg4b,Gel B,MMP-9,pro-MMP-9
<12%
96 Tests
Supernatant
Colorimetric
HRP
50pg/mL to 18000pg/mL
Pro-MMP-9 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Streptavidin-HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of pro-MMP-9 in Samples Using a Microplate Reader
17395
ELISA Kit
RUO
100 μL per sample after dilution
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2,
96 Assays
4 hr. 45 min.
Solid-phase sandwich ELISA
Mouse
Bone Research
RP23-61O3.10-001, AW743869, B/MMP9, Clg4b, MMP-9, pro-MMP-9
<10%
Mouse
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