Invitrogen™ MCP-5 Mouse Instant ELISA™ Kit

Product Code. 15561967

Brand: Invitrogen™ BMS6007INST

Description

Includes

• Aluminum pouch(es) with a Microwell Plate coated with Polyclonal Antibody to mouse MCP-5, Biotin-Conjugate (anti-mouse MCP-5 polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
• Aluminum pouch(es) with a mouse MCP-5 Standard curve (coloured)
• Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Sample Diluent (Use when an external predilution of the samples is needed)
• Stop Solution (1M Phosphoric acid)
• Adhesive Plate Covers

Instant ELISA Kit

The Mouse Moncyte Chemoattractant Protein 5 (Ms MCP-5) ELISA quantitates Ms MCP-5 in mouse serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Ms MCP-5. Principle of the method The Mouse MCP-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

Specifications

• Accession Number: Q62401
• Assay Range: 15.6-1,000 pg/mL
• Assay Sensitivity: 2.3 pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 20293
• Gene Symbol: MCP-5,Scya12
• Interassay CV: 10.7%
• Intraassay CV: 6.7%
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Storage Requirements: -20°C
• Synonym: CCL12, Monocyte chemotactic protein 5, SCYA12
• Target: Mouse
• Test Time: 20 min.
• Total Assay Time: 3 hr. 10 min.

For Research Use Only