Gibco™ Mouse M-CSF Recombinant Protein, PeproTech®

Product Code.	Quantity	unitSize
17882003	50 μg	50µg
17812423	100 μg	100µg
17872233	10 μg	10µg
17812133	1 mg	1mg
17812113	250 μg	250µg
17822333	2 μg	2µg
17862003	500 μg	500µg

Product Code. 17882003 Supplier Gibco™ Supplier No. 3150250UG

Description

Recombinant Protein

Recombinant Murine M-CSF is a 36.4 kDa homodimeric protein consisting of two 156 amino acid polypeptide subunits. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

Spécification

• Accession Number: P07141
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 12977
• Molecular Weight (g/mol): 36.4 kDa
• Name: Mouse M-CSF
• Quantity: 50 μg
• Source: E. coli
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: C87615; colony stimulating factor 1; colony stimulating factor 1 (macrophage); colony-stimulating factor-1 splice variant; CSF1; CSF-1; Csfm; H-MCSF; lanimostim; macrophage colony stimulating factor 1; macrophage colony stimulating factor alpha; macrophage colony stimulating factor beta; macrophage colony-stimulating factor; macrophage colony-stimulating factor 1; macrophage colony-stimulating factor beta; macrophage-colony stimulating factor alpha; MCSF; MCSF alpha; MCSFBETA; MGC31930; M-MCSF; op; osteopetrosis; Processed macrophage colony-stimulating factor 1
• Common Name: M-CSF
• Gene Symbol: Csf1
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.