Mouse IL-7, Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8071-62

Product Code. 15597486

Description

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Description: Mouse IL-7 (also called Lymphopoietin 1, LP-1), a pre-B cell growth factor, is an ∽25kDa factor produced by bone marrow and thymic stromal cells, and spleen cells. IL-7 is a stromal cell-derived growth factor for progenitor B cells and T cells. The main population in the thymus responsive to IL-7 is CD4^-CD8^-. It also stimulates proliferation and differentiation of mature T cells and NK cells. Applications Reported: Recombinant mouse IL-7 is biologically active. Applications Tested: The ED50 of this protein, as measured by 2E8 cell proliferation assay, is less than or equal to 1.6ng/mL. This corresponds to a specific activity of greater than or equal to 6.3 x 10e5 Units/mg. Source: E. coli expressed amino acids Glu 26-Ile 154 of mature mouse IL-7 accession # NM_008371. Bioactivity: The ED50 of this protein, as measured by 2E8 cell proliferation assay, is less than or equal to 1.6ng/mL. This corresponds to a specific activity of greater than or equal to 6.3 x 10e5 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The methionylated polypeptide has a predicted molecular mass of 15,028. The DTT reduced protein migrates as a 15kDa polypeptide. The non-reduced protein has slightly higher mobility on non-reducing SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Accession Number: P10168
• For Use With (Application): Cytokine Bioassay, Control, Functional Assay
• Gene ID (Entrez): 16196
• Name: Mouse IL-7
• Purification Method: SDS-PAGE
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Gene Symbol: Il7
• Specific Reactivity: Mouse
• Cross Reactivity: Ms
• Recombinant: Recombinant
• Protein Subtype: Recombinant

• Concentration: 0.1mg/mL
• Formulation: protein with 0.1M glycine and no preservative; pH 3.0
• Molecular Weight (g/mol): The methionylated polypeptide has a predicted molecular mass of 15,028. The DTT reduced protein migrates as a 15kDa polypeptide. The non-reduced protein has slightly higher mobility on non-reducing SDS-PAGE.
• pH Range: 3.0
• Quantity: 5μg
• Storage Requirements: -80°C
• Structural Form: E. coli expressed amino acids glu 26-ile 154 of mature mouse IL-7 accession # NM_008371
• Common Name: Mouse IL-7
• Biological Activity: The ED50 of this protein, as measured by 2E8 cell proliferation assay, is less than or equal to 1.6ng/mL. This corresponds to a specific activity of greater than or equal to 6.3 x 10⁵ Units/mg.
• Conjugate: Unconjugated
• Species: E. coli
• Form: Liquid
• Purity or Quality Grade: Affinity chromatography

For Research Use Only.