Proteintech™ Mouse IL-6 ELISA Kit

Product Code. 16801606

Brand: Proteintech™ KE1000796T

Description

KE10007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL6.

• Interleukin-6 (Il-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. Il-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule.
• Il-6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation.
• It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS.
• Il-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction.
• Il-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, Il-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
• The assay recognizes mouse IL6. An antibody specific for mouse IL6 has been pre-coated onto the microwells.
• The mouse IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL6 is added to detect the captured mouse IL6 protein.
• For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
• Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Specifications

• Accession Number: NP_112445
• Assay Range: 15.6 to 1000 pg/mL
• Assay Sensitivity: 3.8 pg/mL
• Certifications/Compliance: ISO 13485
• Detection Method: Sandwich ELISA, Double Antibody
• Immunoassay Kit Format: Coated ELISA Kit
• Sample Type: Plasma, Serum
• Substrate: DAB
• Target Species: Mouse
• Gene ID (Entrez): 16193
• Host Species: Mouse
• Immunoassay Kit Grade: Solid
• Interassay CV: <10%
• Intraassay CV: <10%
• Shipping Condition: Blue ice
• Storage Requirements: -20°C to +4°C
• Total Assay Time: 4.5 hr.
• UniProt ID: P08505

Safety and Handling

Product Identifier

• 96 TEST Mouse IL-6 ELISA Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 2
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 1
• ACUTE AQUATIC Acute 1
• Carcinogenicity Category 1A
• Germ cell mutagenicity Category 2
• Reproductive toxicity Category 1B
• Respiratory sensitiser Category 1
• Skin sensitiser Category 1

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.
• H341-Suspected of causing genetic defects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.

Supplemental information

• MIXTURE LIST-Contains : Tetramethylbenzidine, sulphuric acid