Invitrogen™ IL-23 Mouse ELISA Kit

For the quantitative detection of mouse IL-23.

Brand:  Invitrogen™ BMS6017

Product Code. 15561977

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-23
Biotin-Conjugate anti-mouse IL-23 monoclonal antibody
Streptavidin-HRP
Mouse IL-23 Standard lyophilized, 4000pg/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 that is disulfide-linked with to two subunits, a p19 subunit unique to IL-23, and a p40 subunit that is shared with IL-12. IL-23 interacts with IL-12Rbeta1 and a beta2-like receptor subunit with STAT4 binding domain, IL-23R. IL-23 is secreted by activated mouse and human dendritic cells and is involved in the inflammation process and associated with autoimmune diseases. Mouse IL-23 was found not to induce significant amounts of IFN-gamma, induces strong proliferation of memory T cells (but not naive T cells), and activate mouse memory T cells to produce the proinflammatory cytokine IL-17. On the other hand, human IL-23 induces proliferation of memory T cells, induces moderate levels of IFN-gamma, and is produced by naive and memory T cells. IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance, and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines. IL-23 interacts with the receptors IL-12R beta1 and IL-23R to activate the Jak-Stat signaling cascade. IL-23 has been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.

Specifications

• Accession Number: Q9EQ14
• Conjugate: Biotin
• Gene ID (Entrez): 83430
• Product Type: ELISA Kit
• Sensitivity: 16.0 pg/mL
• Sample Volume: Plasma, 50 μL|Serum, 50 μL|Supernatant, 50 μL
• Test Time: 1 hr. 20 min.
• Target: Mouse
• Synonym: JKA3 induced upon T cell activation, SGRF
• Intraassay CV: 8.3%
• Target Species: Mouse
• Label or Dye: HRP

• Assay Range: 31.3-2,000 pg/mL
• For Use With (Application): ELISA
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Size: 50 μL
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 4 hr. 10 min.
• Gene Symbols: IL-23,p19
• Interassay CV: 2.5%
• Quantity: 96 Tests
• Sample Type: Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader

For Research Use Only