eBioscience™ Mouse Flt3 Ligand, Recombinant Protein

Mouse Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 14-8001-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15577476

Quantity Price
1 £ 158.36 / 25µg
EU Stock 6
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Description and Specification

Specification

Source E. coli
Molecular Weight 20.7kDa
Name Flt3 Ligand
Purification Method SDS-PAGE
Formulation Sterile liquid; 20mM glycine, 150mM NaCl, 1% BSA, pH 3.0
Regulatory Status RUO
Structural Form E. coli expressed amino acids Thr28-Gln189 accession number NM_013520
Gene Symbol FL, FLT-3L, FMS-related tyrosine kinase 3 Ligand, Flk-2 Ligand
Storage Requirements Store at less than or equal to -70°C.
Quantity 25μg
For Use With (Application) Cytokine Bioassays
Conjugate Unlabeled
Endotoxin Concentration Less than 0.05ng/μg cytokine as determined by the LAL assay.
Purity >97%
Biological Activity The ED50 of this protein, as measured by IL-6 induction in M1 cells, is 50-250ng/mL in the presence of 1ng/mL Mouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 104 - 4 x 103 Units/mg.
Accession Number NM_013520
Recombinant Yes
Cross Reactivity Mouse
Product Type Recombinant Protein
Concentration 0.1mg/mL

Flt3 (FMS-related tyrosine kinase 3) Ligand is a growth factor that stimulates the growth and differentiation of early hematopoetic progenitor cells. Flt3 Ligand alone exerts weak biological effects, and instead synergizes with other colony-stimulating factors to induce the proliferation and differentiation of cells of the myeloid and lymphoid lineages. Recent studies have suggested that Flt3 Ligand plays an important role in the development and expansion of dendritic cells in vivo, and that it may influence NK cell survival by this mechanism.

Flt3 Ligand transcripts are detectable in most cell types and tissues, although the protein has only been found in stromal cells and T cells. It is expressed most often as a transmembrane cell surface protein, although processing can yield a soluble, secreted isoform.