Mouse Flt3 Ligand (Flt3L), Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8001-80

Product Code. 15577476

Description

Flt3 (FMS-related tyrosine kinase 3) Ligand is a growth factor that stimulates the growth and differentiation of early hematopoetic progenitor cells. Flt3 Ligand alone exerts weak biological effects, and instead synergizes with other colony-stimulating factors to induce the proliferation and differentiation of cells of the myeloid and lymphoid lineages. Recent studies have suggested that Flt3 Ligand plays an important role in the development and expansion of dendritic cells in vivo, and that it may influence NK cell survival by this mechanism.Flt3 Ligand transcripts are detectable in most cell types and tissues, although the protein has only been found in stromal cells and T cells. It is expressed most often as a transmembrane cell surface protein, although processing can yield a soluble, secreted isoform. Description: Flt3 (FMS-related tyrosine kinase 3) Ligand is a growth factor that stimulates the growth and differentiation of early hematopoetic progenitor cells. Flt3 Ligand alone exerts weak biological effects, and instead synergizes with other colony-stimulating factors to induce the proliferation and differentiation of cells of the myeloid and lymphoid lineages. Recent studies have suggested that Flt3 Ligand plays an important role in the development and expansion of dendritic cells in vivo, and that it may influence NK cell survival by this mechanism. Flt3 Ligand transcripts are detectable in most cell types and tissues, although the protein has only been found in stromal cells and T cells. It is expressed most often as a transmembrane cell surface protein, although processing can yield a soluble, secreted isoform. Applications Reported: Mouse Flt3 Ligand Recombinant Protein is biologically active. Applications Tested: The ED50 of this protein, as measured by IL-6 induction in M1 cells, is 50-250ng/mL in the presence of 1ng/mL Mouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 10e4 - 4 x 10e3 Units/mg. Source: E. coli expressed amino acids Thr28-Gln189 accession number NM_013520. Bioactivity: The ED50 of this protein, as measured by IL-6 induction in M1 cells, is 50-250ng/mL in the presence of 1ng/mL Mouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 10e4 - 4 x 10e3 Units/mg. Endotoxin: Less than 0.05ng/ug cytokine as determined by the LAL assay. Purity: >97% as determined by SDS-PAGE. Molecular Weight: 20.7 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Accession Number: P49772
• For Use With (Application): Cytokine Bioassay
• Gene ID (Entrez): 14256
• Name: Mouse Flt3 Ligand (Flt3L)
• Purification Method: SDS-PAGE
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.05ng/μg cytokine as determined by the LAL assay.
• Gene Symbol: Flt3l
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: E. coli
• Form: Liquid
• Purity or Quality Grade: Affinity chromatography

• Concentration: 0.1mg/mL
• Formulation: glycine buffered saline with 1% BSA and no preservative; pH 3.0
• Molecular Weight (g/mol): 20.7kDa
• pH Range: 3.0
• Quantity: 25μg
• Storage Requirements: -80°C
• Structural Form: E. coli expressed amino acids Thr28-Gln189 accession number NM_013520
• Common Name: Mouse Flt3 Ligand (Flt3L)
• Biological Activity: The ED50 of this protein, as measured by IL-6 induction in M1 cells, is 50-250ng/mL in the presence of 1ng/mL Mouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 10⁴ - 4 x 10³ Units/mg.
• Specific Reactivity: Mouse
• Cross Reactivity: Ms
• Recombinant: Recombinant
• Protein Subtype: Recombinant