eBioscience™ Mouse Flt3 Ligand, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8001-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15578726

Quantity Price
1 £ 256.19 / 100µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Purification Method SDS-PAGE
Purity >97%
Recombinant Yes
Regulatory Status RUO
Name Flt3 Ligand
Conjugate Unlabeled
Cross Reactivity Mouse
Quantity 100μg
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Source E. coli
Product Type Recombinant Protein, Carrier-Free
Molecular Weight 20.7kDa
Accession Number NM_013520
Storage Requirements Store at less than or equal to -70°C.
For Use With (Application) Cytokine Bioassays
Biological Activity The ED50 of this protein, as measured by IL-6 induction in M1 cells, is 50-250ng/mL in the presence of 1ng/mL Mouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 104 - 4 x 103 Units/mg.
Structural Form E. coli expressed amino acids Thr28-Gln189 accession number NM_013520
Gene Symbol FL, FLT-3L, FMS-related tyrosine kinase 3 Ligand, Flk-2 Ligand
Formulation Sterile liquid: 20mM glycine, 150mM NaCl, pH 3.0; 0.22μm filtered.
Concentration 0.5mg/mL

Flt3 (FMS-related tyrosine kinase 3) Ligand is a growth factor that stimulates the growth and differentiation of early hematopoetic progenitor cells. Flt3 Ligand alone exerts weak biological effects, and instead synergizes with other colony-stimulating factors to induce the proliferation and differentiation of cells of the myeloid and lymphoid lineages. Recent studies have suggested that Flt3 Ligand plays an important role in the development and expansion of dendritic cells in vivo, and that it may influence NK cell survival by this mechanism.

Flt3 Ligand transcripts are detectable in most cell types and tissues, although the protein has only been found in stromal cells and T cells. It is expressed most often as a transmembrane cell surface protein, although processing can yield a soluble, secreted isoform.