missing translation for 'onlineSavingsMsg'
Learn More

Human M-CSF, Invitrogen™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8789-62

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15577546

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
View alternative products »


Description

Description

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells. Description: Human Macrophage Colony Stimulating Factor (M-CSF, also called CSF-1) is a ∽36 kDa disulfide-linked homodimer produced by a variety of cells including fibroblasts, macrophages, bone marrow stromal cells and endothelial cells. M-CSF regulates macrophage survival, proliferation and differentiation. M-CSF also enhances macrophage killing of tumor cells and microorganisms and regulates cytokine production from macrophages. Applications Reported: Recombinant human M-CSF is biologically active and can promote proliferation of M-NFS-60 cells in culture. Applications Tested: The bioactivity of this protein, as determined in a cell proliferation assay on M-NFS-60 cells, is 3ng/mL. This corresponds to a specific activity of 3.3 X 10e5 Units/mg. Source: Human M-CSF Glu34-Ser190 accession &Num; NM-172211 expressed in insect cells with a C-terminal 6X His tag. Bioactivity: The bioactivity of this protein, as determined in a cell proliferation assay on M-NFS-60 cells, is 3ng/mL. This corresponds to a specific activity of 3.3 X 10e5 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Storage and handling: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2um post-manufacturing filtered.
Specifications

Specifications

P09603
Control, Functional Assay
PBS with 1% BSA and no preservative; pH 7.2
36kDa
7.2
Affinity chromatography
Insect Cells
RUO
Recombinant
Human M-CSF
Control, Functional Assay
Recombinant Protein
Unlabeled
Insect cells
0.1mg/mL
Liquid
1435
Human M-CSF
SDS-PAGE
5μg
-80°C
Human M-CSF Glu34-Ser190 accession # NM-172211 expressed in insect cells with a C-terminal 6X His tag.
Less than 0.01ng/μg cytokine as determined by the LAL assay.
CSF1
The bioactivity of this protein, as determined in a cell proliferation assay on M-NFS-60 cells, is 3ng/mL. This corresponds to a specific activity of 3.3 X 105 Units/mg.
Human
Hu
Recombinant
Videos
Documents