Thermo Scientific™ Pierce™ Mouse IL-12 (total) ELISA Kits

Measure levels of total interleukin-12 (p40 + p70) in mouse serum, plasma and culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ Pierce™ EMIL12TOT2

Product Code. 10371204

Description

Assay IL-12 p40 + p70 (total mouse interleukin-12) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-12 (total) ELISA Kits.

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 47 to 3000pg/mL

• Sensitivity: < 12pg/mL

• Precision: Dependent upon sample type (generally, intra- and inter-assay CV < 10%)

• Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, human IL-12, or pig IL-12

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

• Assay Range: 47 to 3000pg/mL
• Description: Mouse IL-12 Total (p70, p40) ELISA Kit
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• Sufficient For: 2 x 96 microplate wells
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 2 Plate Kit
• Target: Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)

• Assay Sensitivity: <12pg/mL
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1a, RANTES or TNFa, human IL-12, or pig IL-12
• Kit Contents: TMB Substrate Solution, 2 x 12mL, Adhesive Plate Covers, 12 ea, Anti-Mouse IL-12 Total Precoated 96-well Strip Plate, 2 ea, Streptavidin-HRP Concentrate, 2 x 75µL, Stop Solution, 2 x 13mL, Standard Diluent, 2 x 20mL, Lyophilized Recombinant Mouse IL-12 Standard, 4 vials, HRP Dilution Buffer, 2 x 13mL, 30X Wash Buffer, 2 x 50mL, Biotinylated Antibody Reagent, 2 x 12mL
• Precision: Dependent upon sample type (generally, intra- and inter-assay CV <10%)
• Sample Volume: 50 μL
• Total Assay Time: 3 hr.