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Human Glial Cell Derived Neurotrophic Factor (GDNF), Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8506-80

Code : NEW

Additional Details : Weight : 0.09500kg

Product Code. 15597536

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Description

Description

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Description: Glial Cell derived Neurotrophic Factor (GDNF), a member of the TGFβ superfamily, is a neurotrophic factor that promotes the survival of various neuronal populations in both the central and peripheral nervous systems during their development. Neuronal subpopulations affected by GDNF include motor neurons, midbrain dopaminergic neurons and Purkinje cells. GDNF is conserved across mammalian species with human and rat orthologs sharing approximately 93% amino acid sequence identity. E. coli-produced recombinant human GDNF is a disulfide-linked, non-glycosylated homodimer composed of two 135 amino acid polypeptides. Applications Reported: Recombinant human GDNF is biologically active. Applications Tested: This recombinant human GDNF has been tested in bioassays by rat C6 cell proliferation. The ED50, calcμLated by dose-dependent induction of rat C6 cell proliferation, is 1μg/mL, corresponding to a specific activity of 1x10e3 units/mg. Source: E. coli expressed amino acids Ser78-Ile211 accession # CAG46721. Bioactivity: The ED50, calculated by dose-dependent induction of rat C6 cell proliferation, is 1ug/mL, corresponding to a specific activity of 1x10e3 units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 30kDa (homodimer). Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

P39905
Control, Functional Assay
Cytokine Bioassays
PBS with 1% BSA and no preservative; pH 7.2
30kDa (homodimer)
7.2
Affinity chromatography
E. coli
RUO
Less than 0.01ng/μg cytokine as determined by the LAL assay.
GDNF
Recombinant Protein
Unlabeled
E. coli
Recombinant
0.1mg/mL
Liquid
2668
Human Glial Cell Derived Neurotrophic Factor (GDNF)
SDS-PAGE
25μg
-80°C
E. coli expressed amino acids Ser78 - Ile211 Accession # CAG46721
Human Glial Cell Derived Neurotrophic Factor (GDNF)
The ED50 , calculated by dose-dependent induction of rat C6 cell proliferation, is 1ug/mL, corresponding to a specific activity of 1x103 units/mg.
Human
Hu
Recombinant
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