Annexin V, Recombinant Protein, PE, eBioscience™

Product Code. 15551917

Brand: Invitrogen BMS306PE100

Description

Recombinant Protein

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. Description: Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantly located along the cytosolic side of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution in the phospholipid bilayer and translocates to the extracellular membrane, which is detectable with fluorescently labeled Annexin V. In early stages of apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining (PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry. Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V is associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc). Purity: Annexin V is >89% pure as demonstrated by SDS-gel electrophoresis and reverse phase HPLC. The fluorochrome phycoerythrin (PE) shows an excitation maximum of 495 to 564 nm and an emission maximum of 576 nm. Annexin V conjugated to PE is suitable for the detection of PS and allows simple secondary labelling by staining, for example, the membrane surface proteins with a monoclonal antibody for further cellular characterization.

Specifications

• Accession Number: P08758, P48036, P14668
• Concentration: 5μL/Test
• For Use With (Application): Flow Cytometry
• Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.4
• Gene ID (Entrez): 308
• Molecular Weight (g/mol): 35.8kDa
• Name: Annexin V
• pH Range: 7.4
• Purification Method: SDS-gel electrophoresis and reverse phase HPLC
• Quantity: 100 Tests
• Source: E. coli
• Storage Requirements: 4°C, do not freeze
• Regulatory Status: RUO
• Structural Form: E. Coli
• Common Name: Annexin V
• Gene Symbol: ANXA5
• Biological Activity: Annexin V exhibits anti-phospholipase activity and binds to phosphatidylserine (PS).
• Product Type: Recombinant Protein
• Specific Reactivity: All
• Conjugate: PE Labeled
• Cross Reactivity: All
• Species: E. coli
• Recombinant: Recombinant
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: Affinity chromatography