eBioscience™ Annexin V, PE, Recombinant Protein

Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: BMS306PE/100

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Product Code. 15551917

Quantity Price
1 £ 136.19 / 100 tests
Estimated Shipment date
from Supplier 03-04-2017
Add to basket


Description and Specification

Specification

Purification Method SDS-gel electrophoresis and reverse phase HPLC
Storage Requirements Annexin V-PE is stable at 2-8deg;C as indicated on the label. Do not freeze!
Biological Activity Annexin V exhibits anti-phospholipase activity and binds to phosphatidylserine (PS).
Product Type Recombinant Protein
Formulation Solution containing 50mM TRIS, 100mM NaCl, 1% BSA, 0.02% Sodium Azide, pH 7.4
Structural Form E. Coli
Molecular Weight 35.8kDa
Purity >89%
Recombinant Yes
Name Annexin V
Source E. coli
Gene Symbol Lipocortin V, Endonexin II, Calphobindin I
Regulatory Status RUO
Conjugate PE
Quantity 100 tests
For Use With (Application) Flow Cytometric Analysis

Annexins are a family of calcium-dependent phospholipid-binding proteins, which bindto phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantlylocated along the cytosolic side of the plasma membrane. Upon initiation of apoptosis,PS loses its asymmetric distribution in the phospholipid bilayer and translocates tothe extracellular membrane, which is detectable with fluorescently labeled AnnexinV. In early stages of apoptosis, the plasma membrane excludes viability dyes such aspropidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining(PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss ofcell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptakeof PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identifyapoptotic stages by flow cytometry.