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Invitrogen™ Mouse IL-1 beta ELISA Kits
Measure levels of mouse interleukin-1 beta (IL-1b) in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.
Brand: Invitrogen™ EM2IL1B
Description
Assay IL-1β (mouse interleukin-1 beta) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-1 beta ELISA Kits.
These mouse IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant mouse interleukin-1 beta (IL-1β)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 31.2 to 2000pg/mL
Sensitivity: < 3pg/mL
Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with mouse IL-1α, IL-10, IL-2, IL-4, IL-6, TNFα, GM-CSF; human IL-1β, IL-8, IL-1α; or bovine IL-1β. This ELISA does cross-react with rat IL-1β (12%)
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Specifications
31.2 to 2000pg/mL | |
Mouse IL-1 beta ELISA Kit | |
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum | |
96 microplate wells | |
Absorbance at 450nm minus absorbance at 550nm | |
1 Plate Kit | |
Natural and recombinant mouse interleukin-1 beta (IL-1ß) |
<3pg/mL | |
Colorimetric ELISA (TMB substrate) with Plates | |
No significant cross-reaction with mouse IL-1a, IL-10, IL-2, IL-4, IL-6, TNFa, GM-CSF; human IL-1ß, IL-8, IL-1a; or bovine IL-1ß. This ELISA does cross-react with rat IL-1ß (12%) | |
Stop Solution, 6mL, TMB Substrate Solution, 12mL, Streptavidin-HRP Concentrate, 75µL, Standard Diluent, 12mL, Lyophilized Recombinant Mouse IL-1 beta Standard, 2 vials, HRP Dilution Buffer, 13mL, Biotinylated Antibody Reagent, 12mL, Anti-Mouse IL-1 beta Precoated 96-well Strip Plate, 1 ea, Adhesive Plate Covers, 6 ea, 20X Wash Buffer, 55mL | |
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10% | |
50 μL | |
4 hr. |