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Invitrogen™ Mouse IL-1 alpha ELISA Kits

Measure levels of mouse interleukin-1 alpha (IL-1a) in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ EMIL1A

Code : PN

Additional Details : Weight : 0.45000kg Transport : UN number : 3261 Chem class : 8 Pack group : III

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Product Code. 10209193

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Description

Description

These mouse IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse interleukin-1 alpha (IL-1α)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.6 to 1000pg/mL

  • Sensitivity: < 6pg/mL

  • Precision: Intra-assay CV ≤ 10%; Inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, human IL-1β, or mouse IL-1β, IL-3, IL-4, IL-5, IL-7, IL-10, TNFα, IFNγ, or GM-CSF

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

15.6 to 1000pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Biotinylated Antibody Reagent, 8mL, Anti-Mouse IL-1 alpha Precoated 96-well Strip Plate, 1 ea, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL, HRP Dilution Buffer, 14mL, Streptavidin-HRP Concentrate, 75µL, Lyophilized Recombinant Mouse IL-1 alpha Standard, 2 vials, TMB Substrate Solution, 13mL, Stop Solution, 13mL, Standard Diluent, 12mL
Absorbance at 450nm minus absorbance at 550nm
<6pg/mL
50µL
96 microplate wells
Natural and recombinant mouse interleukin-1 alpha (IL-1a)
Mouse IL-1 alpha ELISA Kit
Complete ELISA kit with coated 96-well plate
Intra-assay CV ≤ 10%; Inter-assay CV ≤ 10%
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human IL-1a, human IL-1ß, or mouse IL-1ß, IL-3, IL-4, IL-5, IL-7, IL-10, TNFa, IFNg, or GM-CSF
3 hr.
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