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Invitrogen™ Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet™ 650, eBioscience™
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Product Code. 17902121
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50 μg
Unit Size:
50µg
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17902121 50 μg 50µg
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Product Code. 17902121 Supplier Invitrogen™ Supplier No. 416473281

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Description

Mouse Monoclonal Isotype Control

The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin. Applications Tested This eBMG2b antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Use at the same concentration as the experimental antibody. Blocking Buffers When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Light sensitivity This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Our internal testing suggests that Brilliant Violet™ 650 (BV650) is not compatible with...

Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
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Specifications

Antigen Mouse IgG2b kappa
Applications Control, Flow Cytometry
Classification Monoclonal
Clone eBMG2b
Concentration 0.2 mg/mL
Conjugate Brilliant Violet 650
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Host Species Mouse
Purification Method Affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Target Species Not Applicable
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Isotype Control
Form Liquid
Isotype IgG2b κ
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