Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 506, eBioscience™, Invitrogen™

Mouse Isotype Control

Brand:  Affymetrix eBioscience 69-4724-82

Product Code. 15500687

Description

Description: This is a monoclonal mouse IgG2a antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This eBM2a antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use the isotype control at the same concentration as the experimental antibody. eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

Specifications

• Antigen: Mouse IgG2a kappa
• Clone: eBM2a
• Conjugate: eFluor 506
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Not Applicable
• Form: Liquid

• Applications: Control, Flow Cytometry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG2a κ

For Research Use Only.