Invitrogen™ Mouse Flt3 Ligand, Recombinant Protein, Carrier-Free

Recombinant Protein

Brand:  Affymetrix eBioscience 34-8001-82

Product Code. 15578726

Description

Flt3 (FMS-related tyrosine kinase 3) Ligand is a growth factor that stimulates the growth and differentiation of early hematopoetic progenitor cells. Flt3 Ligand alone exerts weak biological effects, and instead synergizes with other colony-stimulating factors to induce the proliferation and differentiation of cells of the myeloid and lymphoid lineages. Recent studies have suggested that Flt3 Ligand plays an important role in the development and expansion of dendritic cells in vivo, and that it may influence NK cell survival by this mechanism.

Flt3 Ligand transcripts are detectable in most cell types and tissues, although the protein has only been found in stromal cells and T cells. It is expressed most often as a transmembrane cell surface protein, although processing can yield a soluble, secreted isoform.

Specifications

• Accession Number: NM_013520
• For Use With (Application): Cytokine Bioassay
• Molecular Weight (g/mol): 20.7kDa
• Purification Method: SDS-PAGE
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Biological Activity: The ED50 of this protein, as measured by IL-6 induction in M1 cells, is 50-250ng/mL in the presence of 1ng/mL Mouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 10⁴ - 4 x 10³ Units/mg.
• Conjugate: Unconjugated
• Recombinant: Recombinant

• Concentration: 0.5mg/mL
• Formulation: Sterile liquid: 20mM glycine, 150mM NaCl, pH 3.0; 0.22μm filtered.
• Name: Flt3 Ligand
• Quantity: 100μg
• Storage Requirements: Store at less than or equal to -70°C.
• Structural Form: E. coli expressed amino acids Thr28-Gln189 accession number NM_013520
• Gene Symbol: FL, FLT-3L, FMS-related tyrosine kinase 3 Ligand, Flk-2 Ligand
• Product Type: Recombinant Protein, Carrier-Free
• Cross Reactivity: Mouse
• Purity or Quality Grade: >97%