Invitrogen™ Mouse Flt3 Ligand, Recombinant Protein, Carrier-Free

Product Code. 15578726

Brand: Invitrogen™ 34800182

Description

Recombinant Protein

Flt3 (FMS-related tyrosine kinase 3) Ligand is a growth factor that stimulates the growth and differentiation of early hematopoetic progenitor cells. Flt3 Ligand alone exerts weak biological effects, and instead synergizes with other colony-stimulating factors to induce the proliferation and differentiation of cells of the myeloid and lymphoid lineages. Recent studies have suggested that Flt3 Ligand plays an important role in the development and expansion of dendritic cells in vivo, and that it may influence NK cell survival by this mechanism.

Flt3 Ligand transcripts are detectable in most cell types and tissues, although the protein has only been found in stromal cells and T cells. It is expressed most often as a transmembrane cell surface protein, although processing can yield a soluble, secreted isoform.

Specifications

• Accession Number: NM_013520
• Concentration: 0.5mg/mL
• For Use With (Application): Cytokine Bioassay
• Formulation: Sterile liquid: 20mM glycine, 150mM NaCl, pH 3.0; 0.22μm filtered.
• Molecular Weight (g/mol): 20.7kDa
• Name: Flt3 Ligand
• Purification Method: SDS-PAGE
• Quantity: 100 μg
• Source: E. coli
• Storage Requirements: Store at less than or equal to -70°C.
• Regulatory Status: RUO
• Structural Form: E. coli expressed amino acids Thr28-Gln189 accession number NM_013520
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Gene Symbol: FL, FLT-3L, FMS-related tyrosine kinase 3 Ligand, Flk-2 Ligand
• Biological Activity: The ED50 of this protein, as measured by IL-6 induction in M1 cells, is 50-250ng/mL in the presence of 1ng/mL Mouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 10⁴ - 4 x 10³ Units/mg.
• Product Type: Recombinant Protein, Carrier-Free
• Conjugate: Unconjugated
• Cross Reactivity: Mouse
• Recombinant: Recombinant
• Purity or Quality Grade: >97%