Invitrogen™ Mouse Eotaxin ELISA Kit

Product Code. 15218746

Brand: Invitrogen™ BMS6008

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse Eotaxin
Biotin-Conjugate anti-mouse Eotaxin polyclonal antibody
Streptavidin-HRP
Mouse Eotaxin Standard lyophilized, 4ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Mouse Eotaxin(CCL11) ELISA quantitates Ms Eotaxin in mouse serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Eotaxin. Principle of the method The Mouse Eotaxin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil specific chemokine assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.

Specifications

• Accession Number: P48298
• Assay Range: 31.3 to 2,000 pg/mL
• Assay Sensitivity: 3.6 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 20292
• Gene Symbol: Scya11,eotaxin
• Interassay CV: 9.4%
• Intraassay CV: 5.6
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Small-inducible cytokine A11, CCL11
• Target: Eotaxin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 10 mins