Gibco™ Mouse CXCL13 (BLC/BCA-1) Recombinant Protein

Product Code. 10425903

Brand: Gibco™ PMC1614

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in the secondary lymphoid organs. It preferentially promotes the migration of B lymphocytes (both B-1 and B-2) to the follicles of the spleen, lymph nodes, and Peyer's patches. It is produced by macrophages, and in mouse the highest constitutive producers include large peritoneal macrophages, Kuppfer cells and lung macrophages (both CD11c+ and CD11c-). The cellular ligand for CXCL13 is CXCR5 also known as BLR-1 (Burkitt's Lymphoma Receptor 1). CXCR5 is expressed on B cells, Tfh cells and, to a lower degree, some memory T cell populations. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL). CXCL13 is also a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.

Specifications

• Accession Number: O55038
• For Use With (Application): Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 55985
• Molecular Weight (g/mol): 9.5 kDa
• Name: Mouse CXCL13 (BLC/BCA-1)
• Purification Method: Purified
• Quantity: 20 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: 4631412M08Rik; Angie; ANGIE2; B cell-attracting chemokine 1; b lymphocyte chemoattractant; BCA1; BCA-1; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLR1L; B-lymphocyte chemoattractant; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chem; chemokine (C-X-C motif) ligand 13; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); CXC; CXC chemokine BLC; CXC motif chemokine; CXC motif chemokine 13; C-X-C motif chemokine 13; C-X-C motif chemokine ligand 13; CXCL; Cxcl13; SCYB13; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); small inducible cytokine subfamily B (Cys-X-Cys), member 13; small-inducible cytokine B13
• Common Name: CXCL13
• Gene Symbol: Cxcl13
• Biological Activity: Determined by its ability to chemoattract human B cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Sequence: ILEAHYTNLK CRCSGVISTV VGLNIIDRIQ VTPPGNGCPK TEVVIWTKMK KVICVNPRAK WLQRLLRHVQ SKSLSSTPQA PVSKRRAA
• Content And Storage: -20°C
• Activity: Determined by its ability to chemoattract human B cells.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Chemokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Other Proteins
• Research Category: Oncology
• Product Line: Gibco
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC