Proteintech™ Mouse CXCL1 ELISA Kit

Product Code. 16801616

Brand: Proteintech™ KE1001996T

Description

KE10019 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CXCL1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CXCL1.

• The assay recognizes mouse CXCL1. An antibody specific for mouse CXCL1 has been pre-coated onto the microwells.
• The mouse CXCL1 protein in samples is captured by the coated antibody after incubation.
• Following extensive washing, another antibody of biotinylated specific for mouse CXCL1 is added to detect the captured mouse CXCL1 protein.
• For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
• Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
• CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells.
• CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis.
• This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

Specifications

• Accession Number: NP_032202
• Assay Range: 31.25 to 1000 pg/mL
• Assay Sensitivity: 6.5 pg/mL
• Certifications/Compliance: ISO 13485
• Detection Method: Sandwich ELISA, Double Antibody
• Immunoassay Kit Format: Coated ELISA Kit
• Sample Type: Plasma, Serum
• Substrate: DAB
• Target Species: Mouse
• Gene ID (Entrez): 14825
• Host Species: Mouse
• Immunoassay Kit Grade: Solid
• Interassay CV: <10%
• Intraassay CV: <10%
• Shipping Condition: Blue ice
• Storage Requirements: -20°C to +4°C
• Total Assay Time: 4.5 hr.
• UniProt ID: P12850

Safety and Handling

Produktidentifikator

• 96TEST Human TIM-1/HAVCR1 ELISA Kit

Signalwort

• Warning

Gefahrenkategorie

• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Gefahrenhinweis

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.

Sicherheitshinweis

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P234-Keep only in original container.
• P264-Wash skin thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P330-Rinse mouth.
• P362-Take off contaminated clothing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Ergänzende Informationen

• MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine