Invitrogen™ Mouse Complement C5b-9 (soluble) ELISA Kit

Product Code. 30245133

Brand: Invitrogen™ EEL207

Description

ELISA

The complement system is a fundamental part of innate immunity, consisting of over 45 genes that encode various complement components, regulators, and receptors. The complement C5b-9 complex, also known as the Membrane Attack Complex (MAC), plays a crucial role in the immune defense mechanism. It includes the sequential assembly of proteins C5b, C6, C7, C8, and multiple copies of C9. This complex forms transmembrane channels that puncture the cell membrane of target cells, leading to cell lysis. Specifically, MAC has significant implications in mediating cell death in both pathogenic organisms and cancer cells. The genes involved are clustered across the human genome, mainly organized in three significant loci. The understanding of the C5b-9 complex's function extends beyond its antimicrobial roles to include its interactions with tumor cells, where it can induce cell death and influence cancer progression.

Specifications

• Accession Number: P06683
• Assay Range: 0.31 to 20 ng/mL
• Assay Sensitivity: 0.19 ng/mL
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: ELISA Microplate (Dismountable)
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 109828, 12274, 12279
• Gene Symbol: C6, C7, C9
• Interassay CV: <10
• Intraassay CV: <10
• Kit Contents: Micro ELISA Plate (Dismountable), Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma: 100 μL, Serum: 100 μL
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Complement C5b-9
• Test Time: 1 hr. 20 min.
• Total Assay Time: 3 hr. 30 min.