Invitrogen™ Mouse Clusterin ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EM18RBX10

Product Code. 17788053

Description

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Specifications

• Accession Number: Q06890
• Assay Sensitivity: 0.1 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Clu
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min

• Assay Range: 0.1 to 24 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): 12759
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate|Standard|Assay Diluent concentrate|Biotinylated Detection Antibody|SAV-HRP|Wash Buffer|Chromogen|Stop Solution|Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Serum, 50 μL|Plasma, 50 μL|Supernatant, 50 μL
• Target: Apolipoprotein J
• Total Assay Time: 4 hr 45 min