Invitrogen™ Mouse Clusterin ELISA Kit

Mouse Clusterin quantitates mouse Clusterin in serum, plasma, supernatant.

Product Code	Brand	Quantity
17105619	Invitrogen™ EM18RBX5	5 x 96 Tests
17194863	Invitrogen™ EM18RB	96 Tests

Description

Mouse Clusterin quantitates mouse Clusterin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse Clusterin.

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Specifications

• Accession Number: AAG4; Aging-associated gene 4 protein; aging-associated protein 4; AI893575; apo j; Apo J a; Apo J alpha; Apo J b; Apo J beta; Apo J a; Apo J ß; Apo Ja; Apo Jb; APOJ; APO-J; ApoJ a; ApoJ alpha; ApoJ b; ApoJ beta; ApoJ a; ApoJ ß; ApoJa; ApoJalpha; ApoJb; ApoJbeta; Apolipoprotein J; CLI; CLU; CLU1; CLU2; Clusterin; Clusterin alpha chain; Clusterin beta chain; Clustrin; complement cytolysis inhibitor; Complement cytolysis inhibitor a chain; Complement cytolysis inhibitor b chain; complement lysis inhibitor; complement-associated protein SP-40,40; D14Ucla3; DAG; Dimeric acid glycoprotein; HGNC:2095; ku70-binding protein 1; KUB1; MGC24903; Msgp-2; NA1/NA2; RATTRPM2B; SGP2; SGP-2; SP40; SP-40; Sugp-2; Sulfated glycoprotein 2; testosterone repressed prostate message; Testosterone repressed prostate message 2; testosterone repressed prostate message-2; testosterone-repressed prostate message; testosterone-repressed prostate message 2; testostrone-repressed prostate message 2; Trpm2; TRPM-2;
• Assay Sensitivity: 0.1 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Clu
• Intraassay CV: <10%
• Label or Dye: HRP
• Sample Volume: Serum, 50 μL; Plasma, 50 μL; Supernatant, 50 μL
• Target: Apolipoprotein J
• Total Assay Time: 4 hr 45 min

• Assay Range: 0.1 to 24 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): Q06890
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min