Invitrogen™ Monkey IL-2 ELISA Kit

Product Code. 15552087

Brand: Invitrogen™ BMS643/2TWO

Description

ELISA

The Monkey Interleukin 2 (Mk IL-2) ELISA quantitates Mk IL-2 in monkey serum or cell culture medium. The assay will exclusively recognize both natural and recombinant monkey IL-2. Principle of the method The Monkey IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: Q29615,Q865Y1
• Assay Range: 18.8 to 1,200 U/mL
• Assay Sensitivity: 9.1 U/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant, Urine, Whole Blood
• Target Species: Monkey
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 100126754, 102129830
• Gene Symbol: IL-2
• Interassay CV: 5%
• Intraassay CV: 7
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 2 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Amniotic Fluid 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL, Whole Blood 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Aldesleukin, T cell growth factor, TCGF
• Target: IL-2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins