missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ IL-12 p70 Monkey ELISA Kit

For the quantitative detection of monkey IL-12 p70.

Brand:  Invitrogen™ BMS646TEN

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15582087

  • £2250.00 / Pack of 10


Estimated Shipment date
from Supplier 03-03-2020
Add to basket

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey IL-12 p70
Biotin-Conjugate anti-monkey IL-12 p70 monoclonal antibody
Streptavidin-HRP
Monkey IL-12 p70 Standard lyophilized, 500 U/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.
Specifications

Specifications

Q865Y2,Q865Y3
Biotin
100126717, 100126719
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
50 μL
2°C to 8°C
3 hr. 10 min.
IL-12A,Il-12b
4.8%
HRP
Monkey
3.9-250 U/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
2.6 U/mL
Serum, 50 μL|Supernatant, 50 μL
1 hr. 20 min.
Baboon
Interleukin-12 subunit p70, CLMF, NKSF
5%
10 x 96 Tests
Supernatant
Videos
Documents

For Research Use Only