Invitrogen™ Monkey IL-10 ELISA Kit

Product Code. 15592077

Brand: Invitrogen™ BMS6422

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey IL-10
Biotin-Conjugate anti-monkey IL-10 monoclonal antibody
Streptavidin-HRP
Monkey IL-10 Standard lyophilized, 500 U/ml upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Monkey Interleukin 10 (Mk IL-10) ELISA quantitates Mk IL-10 in monkey (baboon, cynomolgus) serum or cell culture medium. The assay will exclusively recognize both natural and recombinant monkey IL-10. Principle of the method The Monkey IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

• Accession Number: P79338
• Assay Range: 3.9-250 U/mL
• Assay Sensitivity: 1.3 U/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Serum, Supernatant
• Target Species: Baboon, Cynomolgus Monkey
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF; EGK_01722
• Gene ID (Entrez): 101024270, 102133450
• Gene Symbol: IL10
• Interassay CV: <10%
• Intraassay CV: <5%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: IL-10, Cytokine synthesis inhibitory factor, CSIF
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2-8°C
• Synonym: TGIF, MCSF III, CSIF, B TCGF
• Target: IL-10
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 10 min