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Invitrogen™ Molecular Probes™ Oregon Green™ 514 Phalloidin

Product Code. 10472282
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Quantity:
300 U
Unit Size:
Each
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Product Code. 10472282

Brand: Invitrogen™ O7465

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Description

High-affinity F-actin probe conjugated to green fluorescent Oregon Green 514 dye

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Oregon Green 514 provides green fluorescence with brightness superior to fluorescein. Oregon Green 514 is a fluorinated analog of fluorescein with greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. Demonstrating very little nonspecific staining, Oregon Green 514 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Oregon Green 514 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Oregon Green 514 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot™ nanocrystals, and Alexa Fluor conjugates including secondary antibodies.

  • Selectively stains F-actin
  • Outstanding fluorescence performance
  • Excitation/Emission: 511/528nm
  • Superior to antibody staining
  • Optimal for fixed and permeabilized samples
  • pH insensitive under physiological conditions

Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cytoskeleton, Organelle Tracing

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Excitation/Emission 511/528
Quantity 300 U
Conjugate Oregon Green 514
Target Actin, Cytoskeleton

For research use only. Not intended for human or animal therapeutic or diagnostic use.

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