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Invitrogen™ EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

Product Code. 10194482
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For Research Use Only. All usage must comply with product instructions.
 
Product Code. 10194482 Supplier Invitrogen™ Supplier No. E33856

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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

For measuring MPO activities and high-throughput screening for MPO-specific inhibitors

Myeloperoxidase (MPO) is unique peroxidase that, in addition to its peroxidation activity, also catalyzes conversion of hydrogen peroxide (H2O2) and chloride (Cl-) to hypochlorous acid (HOCl). The EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit provides assays for determination of both chlorination and peroxidation activities of MPO in solution and in cell lysates.

  • For detection of chlorination, kit includes nonfluorescent 3'-(p-aminophenyl) fluorescein (APF), which is selectively cleaved by hypochlorite (-OCl) to yield fluorescein Peroxidation is detected using nonfluorescent AmplexUltraRed reagent (A36006), which is oxidized by H2O2-generated redox intermediates MPO-I and MPO-II to form fluorescent product
  • EnzChek Myeloperoxidase Activity Assay Kit can be used to continuously detect these activities at room temperature over broad dynamic range (1.5 to 200ng/mL)
  • Kit combines speed (30 minutes), sensitivity and mix-and-read convenience

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species

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Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in refrigerator 2°C to 8°C and protect from light.
Detection Method Fluorescence Intensity
For Use With (Application) Myeloperoxidase (MPO) Activity Assay
Product Type EnzChek Assay Kit
Product Line EnzChek
Quantity 1 Kit
Shipping Condition Wet Ice
Substrate Properties Chemical Substrate
Target Enzyme Myeloperoxidase

For Research Use Only. Not for use in diagnostic procedures.

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