Molecular Probes™ Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 488 Conjugate

Made from a recombinant version of the B subunit only to provide a very high-purity product that is completely free of the toxic A subunit

Overview
Brand: Invitrogen

Manufacturer Part Number: C22841

CHOLERA TOXIN SUBUNIT B (The Alexa Fluor® 488 cholera toxin subunit B

UNSPSC: 12352202

Code: 50

Additional Details:
Additional Details: Weight: 0.00525kg



Product Code. 11564247

Quantity Price
1 £ 606.0 / 500µg
EU Stock 6
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Quantity 500μg
Storage Requirements Store in freezer (-5 to -30°C) and protect from light.

  • Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside GM1, making it powerful tool for retrograde labeling of neurons
  • This tracer has been used in variety of applications, including tracing of rat forebrain afferents, projections of parabrachial region, and neurons of urinary bladder wall
  • When used in neuronal tracing applications, CT-B is typically introduced by pressure injection or by iontophoretic injection into neural tissue
  • At neutral pH, 11.4 kDa B subunit exists as 57 kDa pentamer
  • Lyophilized product can be dissolved in buffer (e.g., PBS) for use
  • More recently, researchers have found that CT-B can be used as marker for lipid rafts, which are membrane microdomains enriched in cholesterol and sphingolipids thought to be important in cell signaling
  • For lipid raft staining, cells are first incubated with fluorescent CT-B, then an anti-CT-B antibody is added to crosslink the CT-B in the lipid rafts into distinct patches on plasma membrane
  • These patches are easily visualized by fluorescence microscopy

  • Label (Ex/Em): Alexa Fluor™ 488 (495/519nm)

Cell Analysis, Cell Structure, Cell Tracing and Tracking, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Lipid Rafts, Microbial Tracking, Neuronal Tracing