MMP9 Monoclonal Antibody (IIA5), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA514228

Product Code. 11345013

Description

MA5-14228 targets MMP-9 (92kDa Collagenase IV) in IHC, IP, and WB applications and shows reactivity with Human and Rat samples. The MA5-14228 immunogen is human native 92kDa MMP-9.

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

Specifications

• Antigen: MMP9
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P14780, P50282
• Gene Symbols: MMP9
• Immunogen: Human native 92kDa MMP-9
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: IIA5
• Conjugate: Unconjugated
• Gene: MMP9
• Gene Alias: 92 kDa gelatinase, 92 kDa type IV collagenase, macrophage gelatinase, matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), matrix metalloproteinase-9, type V collagenase
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 4318, 81687
• Content And Storage: 4° C
• Form: Liquid