Invitrogen™ MMP1-Cell Lysate Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15455073	Invitrogen™ EHMMP1CL	96 Tests
17175509	Invitrogen™ EHMMP1CLX5	5 x 96 Tests

Description

The Human Matrix MetalloProteinase 1 (Hu MMP1) ELISA quantitates Hu MMP1 in human cell and tissue lysate. The assay will exclusively recognize both natural and recombinant Hu MMP1. Principle of the method The Human MMP1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading connective tissue components. Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) is implicated in the invasive potential of malignant tumors. Interstitial collagenase (MMP-1), 72kDa gelatinase A / type IV collagenase (MMP-2), and 92kDa gelatinase B / type IV collagenase (MMP-9) dissolve extracellular matrix (ECM) and may initiate and promote angiogenesis.

Specifications

• Accession Number: P03956
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CLG,CLGN
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 8 pg/mL
• Conjugate: Biotin
• Protein Family: Cell Structure & Organelle Proteins
• Specificity: The antibody pair provided in this kit recognizes human MMP-1.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 4312
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Synonym: CLG, CLGN
• Test Time: 1 hr. 20 min.