Invitrogen™ Human MLK4, GST Tag Recombinant Protein

Product Code. 15970303

Brand: Invitrogen™ A30979

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This MLK4 recombinant human protein (114-420) was expressed in insect cells. MLK4 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service. Validated for use with the LanthaScreen™ Eu Kinase Binding assay Available in multiple pack sizes for convenient use Also available in our SelectScreen Kinase Profiling Service Protein Form: Recombinant, Active, Catalytic The gene accession number for MLK4 is NP_115811.2.

MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas. MLK4 is a negative regulator of TLR4 and does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.

Specifications

• Accession Number: Q5TCX8
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 0.1mM PMSF, 150mM NaCl, 10mM glutathione, 25% glycerol, 0.25mM DTT, 0.1mM EDTA and no preservative; pH 7.5
• Gene ID (Entrez): 84451
• Molecular Weight (g/mol): 60.5 kDa
• Name: Human MLK4, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: BC021891; cDNA sequence BC021891; dJ862P8.3; Kiaa1804; MAP3K21; Mitogen-activated protein kinase kinase kinase 21; Mitogen-activated protein kinase kinase kinase MLK4; mixed lineage kinase 4; mKIAA1804; MLK4; RGD1306091; similar to Mixed lineage kinase 4
• Common Name: MLK4
• Gene Symbol: MAP3K21
• KinaseFamily: MAP Kinases
• Protein Length: 114-420
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 114-420
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Active, Catalytic
• Form: Liquid