PeproTech MIP-3 beta Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  PeproTech 500-P95BBT-25UG

Codice prodotto. 17803303

Descrizione

AA Sequence of recombinant protein: GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3beta (CCL19). Anti-Human MIP-3beta (CCL19)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human MIP-3beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIP-3beta (500-P95B) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3beta. Western Blot: To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P95BBT-1MG will be provided as 2 x 500 μg

CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.

Specifica

• Antigen: MIP-3 beta
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: Ccl19
• Gene Alias: beta chemokine exodus-3; beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine; C-C motif chemokine 19; C-C motif chemokine ligand 19; Ccl19; chemokine (C-C motif) ligand 19; chemokine CCL19; CK beta-11; CKb11; EBI1 ligand chemokine; EBI-1 ligand chemokine; EBI1-ligand chemokine; EBV-induced molecule 1 ligand chemokine; ELC; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MIP3B; MIP-3b; MIP-3-beta; M-MIP-3-beta; SCYA19; small inducible cytokine A19; small inducible cytokine subfamily A (Cys-Cys), member 19; small-inducible cytokine A19
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6363
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q99731
• Gene Symbols: Ccl19
• Immunogen: E.coli-derived, 8.8 kDa Recombinant Human MIP-3beta (CCL19).
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody