Invitrogen™ MIP-3 alpha/CCL20 Cell Lysate Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15387118	Invitrogen™ EHCCL20CL	96 Tests
17894180	Invitrogen™ EHCCL20CLX10	10 x 96 Tests
17165379	Invitrogen™ EHCCL20CLX5	5 x 96 Tests

Description

The Human MIP-3 alpha/CCL20 ELISA quantitates Hu MIP-3α in human cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Hu MIP-3α. Principle of the method The Human MIP-3α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Macrophage Inflammatory Protein-3 alpha (MIP-3 alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3 alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.

Specifications

• Accession Number: P78556
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Inflammation
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 1.5 pg/mL
• Conjugate: Biotin
• Protein Family: Chemokine & Receptors
• Specificity: The antibody pair provided in this kit recognizes human MIP-3 alpha.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 6364
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.