Invitrogen™ MIP-2/CXCL2 Mouse ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15495253	Invitrogen™ EMCXCL2	96 Tests
17115719	Invitrogen™ EMCXCL2X5	5 x 96 Tests

Description

The Mouse MIP-2 (CXCL2) ELISA quantitates Ms MIP-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MIP-2. Principle of the method The Mouse MIP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Macrophage inflammatory protein 2 is a chemokine involved in the pulmonary inflammatory response. MIP-2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. In vitro, it suppresses hematopoietic progenitor cell proliferation.

Specifications

• Accession Number: P10889
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Immunology
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 10 pg/mL
• Conjugate: Biotin
• Protein Family: Chemokine & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 20310
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.