Invitrogen™ MIP-1b (CCL4) Human Instant ELISA™ Kit

For the quantitative detection of MIP-1beta levels in cell culture supernatants, serum and plasma.

Product Code	Brand	Quantity
15551527	Invitrogen™ BMS2030INST	128 Tests
15561527	Invitrogen™ BMS2030INSTTEN	10 x 128 Tests

Description

The Human Macrophage Inflammatory Protein-1beta (CCL4)(Hu MIP-1β) ELISA quantitates Hu MIP-1β in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu MIP-1β. Principle of the method The Human MIP-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Pre-coated plate(s)

Specifications

• Accession Number: P13236
• Assay Sensitivity: 3.0 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 4.9%
• Kit Contents: Pre-coated 96 well Microplate, Adhesive Plate Covers, SAV-HRP, SAV-HRP, Sample Diluent, Standard curve strips, Wash Buffer Concentrate, Wash Buffer Concentrate, Stop Solution, Stop Solution, Lysis Buffer 10x
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 7.8-500 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 6351
• Intraassay CV: 7.2%
• Label or Dye: HRP
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: CCL4, LAG-1, Small inducible cytokine A4, SCYA4
• Test Time: 20 min.

For Research Use Only