Invitrogen™ MICB Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15495063	Invitrogen™ EHMICB	96 Tests
17125509	Invitrogen™ EHMICBX5	5 x 96 Tests

Description

The Human MICB ELISA quantitates Hu MICB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MICb. Principle of the method The Human MICB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Specifications

• Accession Number: Q29980
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 4277
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Biomarker-Related Pathways
• Synonym: DAAP-210H10.1, PERB11.2
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 75 pg/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: MICB
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.