Invitrogen™ MHC Class II (I-A/I-E) Recombinant Rat Monoclonal Antibody (P7/7)

Rat Recombinant Monoclonal Antibody

Brand:  Invitrogen™ MA551851

Product Code. 30088556

Description

This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. This antibody stains MHC class II molecules in a number of rat strains, including PVG, F334, LEW, and BN. However, this antibody is not reactive with RT1a haplotype rats, including Wistar rats. This antibody also cross-reacts with murine I-A and I-E molecules of all haplotypes tested, including H-2b, d, f and k, so can be used in all mouse strains.

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC Class II (I-A/I-E)
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% ProClin 300
• Gene Accession No.: P01910, P01921, P04227, P04228, P04230, P06342, P06343, P06344, P06345, P06346, P14434, P14435, P14436, P14437, P14438, P14483, P23150
• Gene Symbols: H2-Aa, H2-Ab1, H2-Eb1
• Immunogen: This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Clone: P7/7
• Conjugate: Unconjugated
• Gene: H2-Ab1
• Gene Alias: Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1
• Host Species: Rat
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 14960, 14961, 14969
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid