Invitrogen™ Human MET [D1228H], GST Tag, His Tag Recombinant Protein

Product Code. 15478873

Brand: Invitrogen™ PV6208

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Human MET C-terminal fragment, amino acids K956-S1390 (as in GenBank entry NM_000245.2), D1228H mutant, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

MET is a tyrosine kinase receptor for the hepatocyte growth factor. It is linked to functions such as cell proliferation, scattering, morphogenesis, and survival. Ligand binding at the cell surface induces autophosphorylation of MET that provides docking sites for downstream signaling molecules. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

Especificaciones

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM HEPES with 100 mM NaCl, 15 mM glutathione, 20% glycerol, 5 mM DTT and no preservative; pH 7.5
• Molecular Weight (g/mol): 78.8 kDa
• Name: Human MET [D1228H], GST Tag, His Tag
• Quantity: 10 μg
• Common Name: MET (D1228H Mutant)
• Protein Length: 956-1390
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Insect cells
• Form: Liquid