Invitrogen™ Mesothelioma Monoclonal Antibody (ME1)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA2310

Product Code. 11515722

Description

MA2-310 detects a surface membrane glycoprotein preferentially expressed on normal and malignant mesothelial cells from human tissues. This antibody does not detect most lung adenocarcinoma cell lines, or well and moderately differentiated pulmonary adenocarcinomas. MA2-310 has been successfully used in indirect immunofluorescence, immunocytochemistry, immunohistochemistry and solid-phase radioimmunoassay procedures. This antibody does not react in Western blot procedures. Immunohistochemical staining of sarcomatoid mesotheliomas with MA2-310 results in intense staining of mesothelial cells. It has been reported that this antibody reacts with a 200 kDa surface membrane glycoprotein preferentially expressed on normal and malignant mesothelial cells, but does not react with most lung adenocarcinoma cell lines, or well and moderately differentiated pulmonary adenocarcinomas. In studies, this antibody was shown to react with all mesotheliomas examined, with 50%-99% of cells of both pleural and peritoneal mesotheliomas stained. Literature reports have indicated that the antibody does not bind with many common breast (MCF-7, T47D, ZR75-1, BT20, SJC38), ovarian (OVCA429, OVCA432, OVCA434), colon (SW480, SW620, CX1, WIDR, Co112), and renal-cell (FOHN, ZNH1) carcinoma cell lines, nor with several leukemia (K562, U937, CEM) or fibroblast (WI38, R2F) cell lines. Some reactivity has been reported with some pulmonary tumors of squamous-cell and small-cell carcinoma origin, adenocarcino...

Specifications

• Antigen: Mesothelioma
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Immunogen: Mesothelioma cell line SPC111.
• Quantity: 25 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Immunohistochemistry, Radioimmune Assays (RIA), Immunocytochemistry
• Clone: ME1
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1