Invitrogen™ MembranePro™ Functional Protein Expression Kit

Code produit	Quantity	unitSize
17173029	10 reactions kit	10 reactions

Code produit 17173029 Fournisseur Invitrogen™ Code fournisseur A11667

Description

MembranePro™ Functional Protein Expression Kit - 10rxn

For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include:

Proteins are displayed on human (or other mammalian) cell membranes

Cellular quality control and mammalian posttranslational processing helps produce functional protein

Proteins bud off as lipoparticles and are easily collected from the culture medium

Lipoparticles are enriched with expressed receptors

MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:

1. Clone your gene of interest using a TOPO™ vector
2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells
3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

MembranePro™ products are offered in two configurations – as an expression kit (TOPO™ vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.

Spécification

• Type: MembranePro Functional Protein Expression Kit
• Delivery Type: Virus-Like Particles (VLPs)
• Quantity: 10 reactions kit
• Content And Storage: The kit contains:
  •pEF6 V5-His TOPO™ TA Vector Kit for at least 10 TOPO™ cloning reactions (Store at -20°C)
  •3 x10^6 293FT Cells (Store in liquid nitrogen)
  •1 x 75 ml MembranePro™ Precipitation Mix (Store at room temperature)
  •1 x 0.3 mg (1 mg⁄ml) MembranePro™ Reagent (Store at -20°C)
  •3 x 0.75 ml Lipofectamine™ 2000 (Store at +4°C)
  •Manuals
• Selection Agent (Eukaryotic): Blasticidin
• Cell Line: 293FT
• Expression Mechanism: Cell-Based Expression
• High-throughput Compatibility: High-throughput Compatible
• Selection Marker Eukaryotic: BsdR
• Expression System: Mammalian
• Expressed Protein Type: Membrane Protein
• Product Line: MembranePro
• For Use With (Application): Membrane Protein Expression
• Promoter: EF-1α

For Research Use Only. Not for use in diagnostic procedures.