Thermo Scientific™ Melon™ Gel Chromatography Cartridges

Product Code.	Quantity	unitSize
11824161	2 x 1 mL	Pack of 2
11834161	1 cartridge x 5 mL	5mL

Product Code. 11824161 Supplier Thermo Scientific™ Supplier No. 89932

Description

Rapidly purify human, mouse and rabbit IgG from serum and other sources with this simpler alternative to traditional Protein A and Protein G methods.

Thermo Scientific™ Melon Gel IgG Purification Resins and Kits comprise a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Melon Gel IgG Purification Kits enable fast, easy and gentle purification of antibodies from serum and other sample types. Antibody purification with Melon Gel may be performed with gravity-flow columns, spin columns or chromatography cartridges. Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Highlights:

• Simple, one-step protocol – IgG Purification with Melon Gel Resin does not require the binding, washing, and multiple elution steps required by Protein A and Protein G

• Rapid purification – antibody purification from serum is four to six times faster than Protein A or G methods

• High recovery and purity – antibody recovery from serum for many species is greater than 90% with greater than 80% purity

• Minimal dilution – reduced need to concentrate antibody before storage or use

• Robust purification – Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G

• Gentle purification – no harsh antibody elution conditions helps retain IgG activity

• Reusable support – Melon Gel Resin can be used for multiple antibody purifications

• Available in various formats – spin columns, purification kits and chromatography cartridges

Specifications

• Product Type: Chromatography Cartridge
• Product Line: Melon
• Column Type: Proprietary Resin, Affinity
• Format: Chromatography Cartridge
• Stationary Phase: Proprietary Ligand
• Flow Rate: 2 mL/min.
• Purification Target: IgG Antibodies
• Volume (Metric) Bed: 1 mL
• Content And Storage: Upon receipt store at 4°C to 8°C. Do not freeze.
• Quantity: 2 x 1 mL

Frequently Asked Questions (FAQs)

How can I determine when the Melon Gel Support is saturated?

For serum, a volume double the Melon Gel Support volume is the maximum amount that can be processed at one time. To determine if too much sample was processed, examine the purified IgG by SDS-PAGE for additional protein bands.

Can Melon Gel purify chicken IgY?

No. Thermo Scientific Thiophilic Adsorbent (Cat. Nos. 20500 and 44916) can be used to purify IgY from serum. The Chicken IgY Purification Kit (Cat. No. 44918) can be used to purify IgY from egg yolks.

Is the Melon Gel IgG Spin Purification Kit (Cat. No. 45206) a viable alternative to using traditional Protein A and Protein G methods for purification of IgG from serum?

Yes. With the Melon Gel IgG Spin Purification Kit, 90% recovery of IgG with greater than 80% purity are typically observed for a single purification. Although IgA and IgM may be present along with the purified IgG, a high percentage (> 80%) of IgA and IgM is retained on the gel support. This high binding of IgA and IgM to the gel support combined with the fact that IgA and IgM are present in low abundance in serum results in low levels of these antibody molecules in the purified IgG. Here are other advantages of using the Melon Gel IgG Spin Purification Kit over traditional Protein A or Protein G methods:
-Simple, one-step protocol - IgG purification with Melon Gel Resin does not involve binding, washing, and multiple elution steps required by Protein A and Protein G
-Rapid purification - antibody purification from serum is four to six times faster than Protein A or G methods
-Minimal dilution - reduced need to concentrate antibody before storage or use
-Robust purification - Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G
-Gentle purification - No harsh antibody elution conditions helps retain IgG activity
-Regenerable support - Melon Gel Resin can be used for multiple antibody purifications

How many times can I reuse the Melon Gel Support?

The Melon Gel Support can be used up to three times. Performing an ammonium sulfate precipitation on serum samples containing large amounts of non-antibody protein before purification improves purification efficiency and increases reusability.

After Melon Gel purification, must I desalt or dialyze the purified antibody before using the Thermo Scientific Direct IP Kit (Cat. No. 26148)?

No. However, researchers may want to add an equal volume of binding buffer to elevate the pH towards neutral.

Can the Melon Gel Method remove BSA or gelatin from commercially available antibody?

Yes. Dialyze or desalt the antibody solution against the Purification Buffer provided in the kit. The undiluted antibody solution can then be added to the Melon Gel Support using the volumes indicated in the instructions. Tech Tip # 55 (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0055-Melon-Gel-for-BSA.pdf) provides a protocol for this method. Another option is to use Cat. No. 44600 Thermo Scientific Antibody Clean-Up Kit for removing BSA, gelatin and other stabilizer proteins from antibody stock solutions. Sufficient for purifying 10 x 0.5 mL IgG samples.

Are columns provided in the Melon Gel Purification kits?

Microcentrifuge spin cups are provided in the Melon Gel IgG Spin Purification Kit (Cat. No. 45206). No columns are included in the other Melon Gel Kits (Cat. No.s 45212 and 45214) so that researchers can adjust the scale of their purification. A wide variety of disposable columns are available for use with these kits. The product instructions for the Monoclonal Kit include a procedure for using a filter funnel and flask instead of a column for large-scale (e.g., 1 liter purifications.

Does ascites fluid require any special treatment before processing using the Melon Gel Purification Kit?

Yes, ascites samples must be treated with the Ascites Conditioning Reagent (Cat. No. 45219) before antibody purification. See the instructions and other product information for the Monoclonal IgG Purification Kit (Cat. No. 45214).

What formats are available for the Melon Gel Support?

There are three different formats. The Melon Gel IgG Purification Kit (Cat. No. 45212) and Melon Gel IgG Spin Purification Kit (Cat. No. 45206) are for purifying antibodies from serum. The Melon Gel Monoclonal IgG Purification Kit (Cat. No. 45214) is for purifying monoclonal antibodies from cell-culture supernatant and ascites fluid. Ready-to-use prepacked chromatography cartridges are available as two 1 mL columns (Cat. No. 89932) or one 5 mL column (Cat. No. 89933).

How does Melon Gel Purification work?

At physiological pH, Melon Gel Support binds non-antibody proteins in serum, such as albumin and transferrin, allowing the antibody to flow through in a mild buffer suitable for storage and downstream applications.

Can I use the Melon Gel IgG Spin Purification Kit (Cat. No. 45206) to purify IgA and/or IgM from my serum samples?

The Melon Gel IgG Spin Purification Kit is designed for the purification of IgG from serum samples. 90% recovery of IgG with greater than 80% purity are typically observed for a single purification. Although IgA and IgM may be present along with the purified IgG, a high percentage (> 80%) of IgA and IgM is retained on the gel support. This high binding of IgA and IgM to the gel support combined with the fact that IgA and IgM are present in low abundance in serum results in low levels of these antibody molecules in the purified IgG.

Do you offer a kit for antibody purification?

For purification of antibodies, we offer Melon Gel, which binds other proteins from serum, ascites or culture supernatant, allowing the IgG to be collected in the flow-through. As low pH is typically used to elute IgG from Protein A, G, A/G or L, the use of Melon Gel can spare the IgG from exposure to such harsh conditions, which can be particularly important for monoclonal antibodies.

For Research Use Only. Not for use in diagnostic procedures.